Grief-stricken Denise Fergus hit out at the 30-minute flick for "trying to get sympathy" for her son's killers.
The film is a dramatisation of police interviews with Jon Venables and Robert Thompson and has already been nominated for an Oscar.
The pair – then aged ten – shocked the nation when they abducted the toddler and mutilated him before leaving his body on railway tracks in Bootle, Merseyside, in 1993.
Speaking on ITV's Loose Women this morning, Denise blasted: "The first I heard about the film was just before Christmas. They should have got in contact and let us know.
"I'm asking people to boycott it, I don't think it should have been made in the first place, especially without James's family knowing.
"I don't think they had the right to do this. I would like to have it removed from the Oscar list to stop this happening again."
"I don't want to keep reliving the past. I haven't watched it, and I won't. It takes you right back to that day and that's not something I want to do.
"There's a child playing James in it and I don't think I'd be able to see it and see another child running around pretending to be him."
"I wouldn't meet with the director to hear his apology. He's trying to big himself on someone else's grief.
"The way he's done it, he's trying to get sympathy for those two – they don't deserve any.
Director Vincent Lamb admitted on Good Morning Britain this week he hadn't spoken to Denise or James' dad Ralph before making the film.
He claimed he made the film to get a "deeper understanding" of the crime, and the boys who committed it.
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